Custom oligonucleotides for research, diagnostics and therapeutic discovery
Bespoke DNA, RNA and modified oligonucleotides for research, diagnostics and therapeutic discovery, every product verified by UPLC-MS before release. ISO 9001 certified.
Mass and purity checked by UPLC-MS before release
Reviewed by our scientists before it starts
ISO 9001 certified by BSI
Standard orders in under two weeks
Why Assemblon?
Assemblon is run by chemists with decades of experience in oligonucleotide synthesis and a deep understanding of nucleic acid chemistry and its applications. We take a direct consultative approach. You talk to our chemists, who will work with you, scientist to scientist. The chemistry deserves thought before anything is made, and we are here to advance the field, not simply to supply it.
Capabilities at a glance
Synthesis scales
200 nmol, 1 µmol and 16 µmol as standard, with larger scales on request. Order by minimum required yield if you need a specific quantity.
Purification
Desalting, reversed-phase HPLC and anion-exchange HPLC, chosen for the sequence and its modifications.
Backbones and sugars
Phosphorothioate and other backbones; 2′-OMe, 2′-MOE, 2′-fluoro, LNA and other sugars; DNA, RNA and mixed, as gapmer, mixmer or fully modified designs.
Labels and modifications
Any modification available as a phosphoramidite or by post-synthesis conjugation, including dyes, quenchers, biotin, amino and thiol linkers, click handles, lipids, GalNAc and more. If it exists, we can almost certainly make it.
How we will work with you
Tell us what you need
Sequence, scale, modifications and turnaround, by order form, spreadsheet or email. We are happy to meet or talk it through first.
Scientist to scientist
Experts in the field review your order and confirm feasibility, cost and timing. You deal with them directly.
Synthesis to QC
Synthesis, purification, quantification, then QC by UPLC-MS before release.
Ships with a datasheet
Every order is dispatched with a comprehensive datasheet.
Standard orders ship in under two weeks. Larger or more complex orders are scheduled at quotation.
Find the right pathway
All services →For researchers
Standard and modified oligos for academic and industrial benchwork.
For diagnostics
Probe chemistry and qPCR primers for diagnostic assays, with batch traceability.
For therapeutic discovery
ASO chemistry, complex modifications and scale-up for in vivo studies.
For complex projects
Consultation with experts in the field on unusual chemistries and novel modifications.
As standard with every order
Mass confirmed by UPLC-MS
Observed mass compared with the mass calculated from the sequence you ordered.
Purity by UPLC
Checked on a method appropriate to your sequence and modifications, and confirmed against our release criteria. Reported as a conformance statement, not a figure.
Full datasheet
Sequence, modifications, scale, purification, physical form, observed and expected mass, concentration and volume. The full UPLC-MS report, with the chromatogram and deconvoluted mass spectrum, is available on request.
UPLC chromatogram
Deconvoluted mass spectrum
Founded by oligonucleotide chemists
Meet the founders →Assemblon was founded by three chemists who have dedicated their careers to oligonucleotide R&D.
Tom Brown is Emeritus Professor of Nucleic Acid Chemistry at the University of Oxford and co-inventor of Scorpion and HyBeacon probes. Daniel Singleton has a background in novel oligonucleotide chemistry and synthesis. Richard Cunningham's background is in the purification and mass-spectrometric characterisation of modified oligonucleotides.
They are the people who review your synthesis before it starts, and who you talk to if your data raises a question. There is no sales chain to navigate and no automated responses.
The same analysis is available to anyone: oligonucleotides made in-house, in an academic or research lab, or by another supplier. Send us a sample and we will report its intact mass and purity, on the same instruments and methods we use for our own material, with no synthesis order required.
- Duplex and triplex melting analysis
- Peak identification and assignment
- Deconvoluted masses of all peaks at 10% UV area or above
- PDF report with the UPLC and MS spectra
Not sure where to start?
Tell us about your requirements and experimental design before you place an order, and we will help you identify the most suitable approach. The initial consultation is free.